The effect of mutagenic treatments combined with immature embryoculture on the variation frequency of common wheat in R2 generation(M_3R_2)wastested using three wheat varieties.The immature embryo derived from theseeds pre-treated with mutagens(EMS0.2M,r-radiation 30KR,SA 2mM)was inoculated.The results showed that the M_2R_2 treated with EMS hadthe highest variation frequency and proportion of non-segregating variationin R2 generation.The highest useful variation frequency were found inr-radiated material.A...