The basidiocarps of Calvatia gigantea were extracted with water,and precipitated with ethanol.The precipitates were by enzyme and separated by a Sepharose 2B column,to afford a homogeueous fraction,CGII. Antiinflammatory effects of CGlI was shown by depressing the inflammatory edema of ear induced by dimethylbenzene and the joint arthritis of ankle induced by formaldehyde in mice.Analgesic effects of CGII were shown by inhibiting the writhing response(induced by acetic acid)and prolonging the reaction time ...