Actin filament(AF)distribution in Zea mays pollen and Gladiolus gandavensis pollen protoplasts was localized by FITC conjugated phalloidin fluorescence microprobe.The pollen was incubated in Brewbaker and Kwack(BK)medium,and the pollen protoplasts were isolat- ed enzymatically and cultured in K3 medium containing various supplements by a previously reported method.Samples were fixed for 30 min with 1.5% paraformaldehyde dissolved in 0.1 tool/l phosphate buffer(pH 7),half strength of BK elements,1 mol/l EGTA...
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