Large amount of viable mesophyllprotoplasts were isolated enzymaticallyfrom onion(Allium cepa L.).The pro-toplasts grew and divided when culturedin thin liquid layers of MS mediumwith 2,4-D 2,6 BA 0.5 mg/1,mannitol0.5 M,sucrose 2%.Calli were subcultu-red on MS medium without mannitol.After transferring to modified MS me-dium(MS_1,MS_2,MS_3)calli regeneratedbuds or bulblets and regenerated plant-lets had been obtained.
| 
推荐 
点击下列相关研究机构和相关文献作者，可以直接查到这些机构和作者被《中国知识资源总库》收录的其它文献，使您全面了解该机构和该作者的研究动态和历史。
