The main concern in the enzyme engineering has focused on the fabrication of the highly-efficient nano-biohybrid catalysts through the synergistic interfacial assembly of the nanomaterials with enzymes. However, the interfacial assembly often leads to the denaturation of the enzyme active sites or the inaccessibility for the substrate to the active sites, resulting in a remarkable decrease of the bio-activity. The main challenge is to well fabricate nanoscale bio-interfaces based on nanomaterials to achieve...
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