The petroleum hydrocarbon pollution of the soil has imposed a serious threat to the safety of human being health and the ecosystem health. Bioremediation has been the technology of potential for its cost effectiveness. Low bioavailability is the main factor which limits its biodegradation, and could be improved by adding biosurfactants. Biosurfactants can enhance the solubility of petroleum hydrocarbons, improve the properties of the cell surface and therefore increase the mass transfer rate and its bioavai...