The formation of passivation layer is one of the major reasons for the low copper extraction and low extraction rate during bioleaching of chalcopyrite. This thesis focus on the investigation of the formation mechanism of passivation layer during bioleaching of chalcopyrite from four aspects:biology, ecology, electrochemistry and mineralogical, and the investigation results would provide the theoretic evidence to find a way to eliminate or reduce the passivation layer on the mineral surface. At the end, col...