Apple(Malus domestica Boukh.)is one of the most popular fruits in the world. It is of great value to understand the developmental changes of carbohydrates and organic acids and malate metabolism profile in different genotypes of apple fruit for evaluation of apple germplasm and apple breeding. Here we determined carbohydrates and organic acids in different genotypes of apple during fruit development by GC-MS and investigated the relative expression levels of genes involved in malate metabolism with real tim...