This paper studied genetic diversity among 30 wild Eremochloa ophiuroides germplasm resources collected from 12 places in Hunan province by using Inter-Simple Sequence Repeat (ISSR) markers and analyzing morphological characteristics. The results got as follows:(1) Big variation of 30 Eremochloa ophiuroides took place on 12 morphological indexes, among which the variations of root depth, leaf area, length of leaves-hair, stem color and leaf color achieved above 20 percent. (2) The correlation analysis on mo...