A bacterial strain ZJU15, isolated from rice leaf in Hangzhou, China and was identified as Bacillus subtilis ZJU15 according to the conventional physiological biochemistry properties, electron microscope and 16S rDNA sequence analysis. The cell-free supernatant (CFS) of B.subtilis ZJU15 showed antimicrobial activity against several important Gram-positive pathogenic and food-spoilage bacteria, which including Enterococcus feacalis, Listeria monocytogenes, Micrococcus luteus, meticillin-resistant Staphylococ...