Objective To explore the existence of Vasculogenic mimicry in human Retinalblastoma; to explore the effect of hypoxia on inducing retinalblastoma cell to transform to endothelioid cell and form vasculogenic mimicry through three-dimension culture in vitro; and to build shRNA virus vector targeting at hypoxia inducing factor-la using RNAi technology; to observe the effect of hypoxia inducing retinalblastoma cell to transform to endothelioid cell.
Methods 1. To collect human Retinalblastoma sample...