海湾扇贝(Argopecten irradians)不连续分布于美国大西洋沿岸和墨西哥湾沿岸,自1982年以来北部亚种(A. i. irradians)和南部亚种(A. i. concentricus)被先后引进到中国,由于其生长速度快、繁殖周期短和适宜温度范围广的特点,迅速成为我国海水养殖的重要品种。近年来飞速发展的分子标记技术为优良品种的选育注入了新的活力,相对于传统的表型选择来说,标记辅助选择不易受环境的影响,尤其是对于低遗传力性状和后期表达的性状,能增强选择效率,提高选择的准确度,缩短育种周期。本文通过构建海湾扇贝微卫星富集文库获得大量的微卫星DNA序列,筛选多态的微卫星标记构建了海湾扇贝的遗传连锁图谱,并应用复合区间作图法对生长相关性状进行了QTL定位。
本研究利用富集文库-菌落原位杂交法筛选海湾扇贝微卫星DNA,吸附(AC)15和(AG)15探针的尼龙膜捕捉并富集含有微卫星序列的片段,菌落原位杂交结果显示阳性克隆率达到40%,测序比对后获得521个独立的阳性克隆,其中微卫星506个,小卫星15个。微卫星中,完美型248个,占49.0%,非完美型216个,占42.7%;复合型4...
【英文摘要】
The molecular genetic linkage map of bay scallop is essential for the identification and localization of quantitative trait loci (QTL) as well as for the maker-assisted selection program, which is great useful in enhancing breeding efficiency in this aquaculture important species. The purpose of this study includes four aspects. The first is to isolate lots of microsatellite DNA sequences by microsatellite-enrichment libraries. Second is to investigate the level of genetic differentiation between one...