Aflatoxin B_1 (AFB_1) seriously threatened human and animal healthy or life safety. AFB_1 commonly contaminates agricultural commodities, food and animal feedstuffs, which is an important problem all through. Standard of AFB_1 limits and technical barriers to trade (TBT) were set by many countries. TBT led to impeded export of agricultural production and damaged the interests of farmers. Development of high sensitivity test method for AFB_1 determination is important and crucial to break out TBT. ...