Dendrobium huoshanense, a precious wild medicinal plant in China, distributes in Huoshan County, Anhui Province. Polysaccharides isolated from Dendrobium can inhibit the growth of tumor cells and possess immunological activity. Because of a deficiency in the wild source of Dendrobium huoshanense and excessive collection, it has been on the verge of extinction. Tissue culture techniques have been used for the in vitro mass multiplication of Dendrobium huoshanense, but the culture system has not ...