本课题以内蒙古牧区传统乳制品中的乳杆菌为研究对象,从菌株的分离鉴定、生物学特性研究、潜在益生菌的体外筛选、体内生理功能的探讨与验证等方面对其进行了较为系统、全面的研究,以期为传统乳制品中乳杆菌资源的开发利用奠定基础,同时也为益生乳杆菌在功能性食品及微生态制剂中的应用提供微生物菌种和理论支持。具体得出以下结论:
1.从98份乳样中共分离到乳杆菌属细菌53株,除1株为异型发酵外,其余均为同型发酵。其中L. plantarum 30株,L. casei 5株,L. helveticus 5株,L. coryniformis subsp. coryniformis 3株,L. curvatus 3株,L. zeae 2株,L. agilis、L. murinus和L. farciminis各1株,另有2株未能鉴定到种。大多数菌株具有较宽的温度生长范围。样品虽然来源于气候环境、草原类型、畜种不尽相同的三个地区,但其优势菌株均为植物乳杆菌。
2.对53株乳杆菌的潜在益生作用研究发现,在含0.3%牛胆酸钠MRS培养基中生长率超过30%,同时在pH3环境中孵...
【英文摘要】
To establish foundation for exploitation and utilization of lactobacillus resource and to provide strains and principle knowledge for utilizing of beneficial lactobacilli in functional food and microbial ecological agents, Lactobacillus strains which were isolated from traditional dairy products in Inner Mongolia were studied systematically and comprehensively from the following several aspects: isolation and identification of strains, studies on biological properties, in vitro screening of pote...