Monitoring phytoplankton classes and their abundance is a rountine task in marine scientific research. With the frequent occurances of red-tide, there is an urgent need for rapid analysis methods that can provide qualitative and quantitative information. Three dimensional fluorescence spectrum, also termed as excitation-emission matrix (EEM) spectrum, gives total fingerprints information in the whole range of wavelenghths. By using chemomatrics on extracting their spectral features, EEM spectrum is often us...