The transgenic of Phalanopsis , Cymbidium hybridum and Oncidium were studied and the the main results are as follows:(1) Decontamiated-materials can be obtained from unsymbiotic- seed germination, or by inducing from floral stalk, and proliferation of plantlets was achieved on MS media supplemented with 6-BA7.5mg/L+NAA1.0mg/L; 6-BA and NAA can stimulate PLB formation and the effective ruslts can be obtained on MS with 6-BA10.0mg/L+NAA1.0mg/L; The optimal medias were 1/2MS and Hyponex medias for proliferatio...