We report a molecular genetic linkage map for an interspecific F1 hybrid population between Populus deltoides (clone ‘I―69’)×P. euramericana (clone ‘I―45’) by means of two-way pseudo-testcross mapping strategy. A total 462 markers were available for mapping on the maternal tree ‘I-69’, including 164 SSRs, 206 AFLPs, 83 RAPDs, 2 SNPs, 6 ISSRs and one sexual differentiation trait. Twenty nine linkage groups were constructed, map length between 9.2 cM~153.9 cM, which 60.4 cM was average group length. The avera...