One porcine circovirus type 2 (PCV2), originally isolated from a pig affected with postweaning multisystemic wasting syndrome (PMWS), was propagated through porcine kidney cell line PK15. The virus genome amplified by polymerase chain reaction (PCR) was inserted into pMD-18T vector as pMD-PCV. The genomic nucleotide sequence was .found to have homology with other PCV2s deposited in the GenBank database from 93% to 99%. The isolated strain was named PCV2 JXL and registered to GenBank with number AY491310.
...