Acetobacter strain M12 was isolated from membranous vinegar which showed higher and steadier BC production. According to The Common Methods of Determinative Bacterial and Bergey's Manual of Determinative Bacteriology(ninth edition), M12 was characterized Acetobacter xylinum. The optimum fermentation condition of M12 was: 6% of inoculum size , fermentation temperature 30 C, pH 2.5-7.7, culture time 6 days.
By orthogonal design of experiment, the production of BC increased by 40% with the optimized m...