本研究利用AFLP、RAPD和微卫星标记,以一个回交家系[(Miyagi x Hiroshima) x Miyagi]为作图群体,构建了长牡蛎的遗传连锁图谱。
从56对AFLP引物组合中筛选出17对亲本和80只子代个体进行了分离分析。共得到1106个AFLP位点,其中有384个位点在亲本间有多态性,占总位点的34.7%。384个多态标记有349个在子代中产生了分离。母本中有191个分离标记,父本中有158个分离标记。卡方检验显示,有255个标记符合孟德尔1:1分离标记(73.1%),有94个属于偏分离标记(26.9%)。利用经过筛选出27个RAPD单引物和6对RAPD引物组合,共得到338条片段,其中有102 (30.2%)条在亲本间呈多态性。有94条片段在父本或母本中分离。卡方检验显示74(78.7%)个分离标记符合孟德尔1:1遗传比例,20个为偏分离标记。15个在家系中有作图信息的微卫星位点,分别有13个和11个可用于母本和父本的作图。微卫星位点中有大量的无效等位基因(null allele),占所有位点的46.7%,是所有等位基因的11.7%。微卫星的无效等位基...
【英文摘要】
Amplified fragment length polymorphisms (AFLPs), Random Amplified Polymorphic DNA (RAPD) and microsatellite markers were used for genome mapping in the Pacific oyster, Crassostrea gigas Thunberg. Seventeen selected AFLP primer combinations produced 1106 peaks, of which 384 (34.7%) were polymorphic in a backcross family. Among the polymorphic markers, 349 were segregating through either the female or the male parent. Chi-square analysis indicated that 255 (73.1 %) markers segregated in Mendelian r...