本论文对拥有自主知识产权(专利申请号：01108274)的Bt菌Ly30株进行了系统的研究。首先从形态学水平上确定该菌株杀虫晶体蛋白组分及多种形态的伴孢晶体，进而确定其杀虫谱及毒力高低，然后深入到分子水平，鉴定了ICPs基因的种类，并对ICPs基因进行克隆、表达及生物活性测定。主要结果如下：
1．电镜观察Ly30株拥有大菱形、小菱形、方形、不规则形以及镶嵌形等多种形态的伴孢晶体。SDS-PAGE检测其至少拥有2种蛋白组分，分子量分别为135-140kDa和60kDa，也有弱的85kDa和70kDa蛋白表达。生测结果显示，该菌株不仅对小菜蛾、棉铃虫等鳞翅目昆虫有较高的毒力，其LC50分别为0.022 μg／ml和2.252μg／ml，而且还对直翅目、双翅目昆虫表现一定的毒效，如对黄胫小车蝗(Oedaleusinfernalis de Saussure)的校正死亡率为81.82％，对尖音库蚊(Culex pipiens Linnaeus)的LC50为19.8μg／ml。Bt菌株对直翅目昆虫有毒效未见国内报道。
2．CAPS系统鉴定该菌株...
【英文摘要】
In this thesis, the molecular biology of Bacillus thuringiensis Ly30 strain (patent application number: 01108274) were systematically studied. The components of insecticidal crystal proteins and parasporal crystals of Ly30 strain were firstly defined based on morphology. The insecticidal spectrum and toxicities of Ly30 strain were tested. Several ICPs(Insecticidal Crystal Proteins) genes were identified, cloned and expressed, and then the bioassay of the expression products were carried out. The major resea...
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