Isolation and Identification of the Mixture of L-Hydroxyproline Oligo-Peptides by Reversed-Phase High Performance Liquid Chromatography and Electrospray Ionization Tandem Mass Spectrometry
A reversed-phase high performance liquid chromatographic-electrospray ionization tandem mass spectrometric method(RP-HPLC/MS2) was developed for the analysis and identification of a reaction mixture of L-hydroxyproline oligo-peptides.The mixture was separated on a YWG C8 column(10 μm,250 mm×10 mm) using acetonitrile-water containing 0.06% trifluoroacetic acid(2∶98,v/v) as the mobile phase.The separation conditions were optimized.The mixture was analysed with electrospray ionization mass spectrometry(ESI-MS)...