c DNA末端快速扩增 (RACE)技术是快速获得新基因 5′和 3′端未知序列的有效手段 .鉴于新报导的人肿瘤基因 MAGE家族成员之一 MAGE- D1的 c DNA序列不完全 ,从而拟用 RACE技术获得 MAGE- D1的全长 c DNA序列 .结果显示 ,MAGE- D1的 c DNA全长为 2 80 0个碱基 ,编码778个氨基酸 .同时对 RACE技术应用时的一些关键问题进行了讨论 .
【英文摘要】
Rapid amplification of cDNA ends(RACE)is a powerful technique to obtain unknown sequence of 5′ terminal or 3′ terminal part of a new gene.As the cDNA sequence of the newly discovered tumor related gene MAGE D1 is not in full length,RACE was used to study the 5′ terminal of this cDNA.The result showed that the full length cDNA sequence of MAGE D1 is 2800 bp in size and encodes a polypeptide of 778 amino acids.Some key problems were discussed in RACE.
| 
推荐 
点击下列相关研究机构和相关文献作者，可以直接查到这些机构和作者被《中国知识资源总库》收录的其它文献，使您全面了解该机构和该作者的研究动态和历史。
