Since late2010, severe outbreaks of porcine epidemic diarrhea virus (PEDV)infection have reemerged in China Mainland. In order to study the reason of there-emergence, a whole length of S gene, each of12PEDV strains isolated fromdifferent districts, central China from2010to2012, was amplified and cloned byRT-PCR, and then sequenced and analyzed. An indirect ELISA was developed todetection anti-PEDV antibody, which would be suitable for monitoring anti-PEDVantibody and epidemiologic survey of PED. In addition...