Royal jelly(RJ)possesses numerous functional properties to benefit human health.Major royal jelly proteins(MRJPs)as the most characteristic component of royal jelly,has become the focus of current research interests.In this study,royal jelly collected from European honeybees(Apis mellifera)was researched.We aimed to propose a sequential processing strategy to isolate and purify MRJP 1-3 from royal jelly,and a high sensitivity SEC-HPLC was used to identify the purity of separated proteins for the first time....