ObjectiveThis research used the method of density gradient centrifugation and cytospin to enrich tumor cells in peripheral blood.Then,Giemsa staining was utilized to observe cell morphology combined with immunohistochemistry and immunofluorescence to identify whether the captured cells were tumor cells according to the specific molecular on cell surface.Hence,a more simple and convenient method of capture and detecting circulation tumor cells was likely to be established.Methods:1.Enrichment:The method of d...
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