目的:1.建立简便、灵敏的高效液相色谱串联质谱法(LC-MS/MS)测定患者血浆中5-氟尿嘧啶(5-FU)、紫杉醇(PTX)和多西紫杉醇(DTX)的浓度。2.采用免疫比浊法即纳米增强免疫比浊法(nanoparticle immunoassay,NPIA)测定患者血浆5-FU、PTX和DTX浓度。3.比较LC-MS/MS和NPIA两种方法5-FU、PTX和DTX血浆浓度测定结果的相关性。方法:1.LC-MS/MS方法:5-FU:色谱条件:色谱柱:DiamonsilC18柱(150 mm × 4.6 mm,5μm);流动相:0.05%氨水:甲醇(2:98,v/v),在线脱气;流速:0.3 mL/min;柱温:30℃。血浆样本经液液萃取处理后,进样体积为10 μL进行分析。PTX和DTX:色谱条件:色谱柱:Diamonsil C18柱(150 mm × 4.6 mm,5 μm);流动相:0.1%甲酸:乙腈(40:60,v/v),在线脱气;流速:0.6 mL/min;柱温:30℃。血浆样本经沉淀蛋白处理后,进样体积10μL进行分析。2.NPIA方法:5-FU、PTX和DTX血浆样品按一定操作程序(采用专属试剂盒)处理...
【英文摘要】
Objectives:1.Sensitive and simple LC-MS/MS methods were established for determination of 5-fluorouracil(5-FU),paclitaxel(PTX)and docetaxel(DTX)in human plasma,respectively.2.NPIA was used to measure the concentration of 5-FU,PTX and DTX in human plasma,respectively.3.To compare the difference and correlation of the results of the concentration of 5-FU,PTX and DTX between the determinations of LC-MS/MS and NPIA,respectively.Methods:1.LC-MS/MS method:5-FU:5-FU was separated on a Diamonsil C18 column(150 mm × ...
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