N. incisum and N. franchetii are perennial herbs which grow in the Qinghai-Tibet Plateau and the nearby mountain areas. They are Chinese endemic plant which under threaten of extinction. The Tibetan Plateau uplift and the climate fluctuations during Pleistocene ice age have great impact on the speciation process and the evolutionary history of N. incisum and N. franchetii species, we adopted sequences of chloroplast, mitochondria and nuclear gene in 122 individuals from 21 populations in Qinghai, Gansu, Sic...