The activity of three major hemicellulases produced by Piptoporus betulinus in seven different culture media were measured. The enzymatic properties were also partly screened. To investigate the number and structure of lignocellulose-degrading genes in P. Betulinus, and their different regulation ways in wood-degradating process, we induced fungi using different carbon source for 20 days (birch sawdust induced for YLX-B and none for YLX-A), and then analyzed and sequenced the transcriptome of fungi, in orde...