The adults of Dastarcus helophoroides Fairmaire (Coleoptera: Bothrideridae) could livemore than12years, which is a suitable insect to study genes related in aging process.Members of the caspase genes family play a central role in programmed cell death (PCD), andthey also play an important function in the development and cell decline of the organism.Real-time quantitative PCR is one of the advanced technologies to study gene expression,which need suitable reference genes for analyzing expression accurately. ...