Ligand-exchange chromatography (LEC) is a convenient and effective method for aminoacids racemate chiral separation. Immobilized type Ligand-exchange chiral stationary phase(CSP) is synthesized by two steps: First, KH-560react with silica for preparation of KH-560bonded silica (KBS). Afterwards, amino acids are used to react with epoxy group whichpresent in KBS for preparation of Ligand-exchange CSPs. So it is necessary to determineKH-560content in KBS and amino acid content in Ligand-exchange CSPs, respect...