Currently, High Performance Liquid Chromatography (HPLC) technology with the highresolution becomes one of the most commonly used techniques for the protein separation andanalysis, providing effective support for protein conformation and structure study. However,the single patter of HPLC technology still can’t meet the requirements for the separation andanalysis of the mixed commonents to reach the crystal purity. In recent years, a newdule-mode chromatography: Hydrophobic Charge Induction Chromatography (H...