As an important separation and analytical method, high performance liquid chromatography (HPLC) plays an important role in life science, medical science, chemical industry and environmental science. It is of great significance to research, develop and improve chromatographic media, because column is the heart of the HPLC. A lot of advantages, high permeability, porous structure and large specific surface area, make monolithic column have a rapid development, especially in the separation and analysis biomacr...