Tobacco germplasm resoureces are mainly kept by storying seeds in low tempetature, the life ofgermplasm resources could be prolonged, but this can’t stop the change of the genetic integrity. Theseeds aging to a certain degree need regeneration, and the population size is the primary factor. It is ofgreat significance for genetic integrity to study the effect of different population size and seed ageing.N.tabacum germplasm including Honghuadajinyuan, Wankouhongtuyan, Baihuaheiyan, Yunyan87,Heiguzhong and wil...