始兴石斛(Dendrobium shixingense Z. L. Chen, S. J. Zeng et J. Duan)于2010年发现于广东北部始兴县,现仅发现广东及江西交界的少数地区有分布,适生海拔约400-600m。近年来,野生石斛资源遭到掠夺式采集,已经面临濒危灭绝的境况。
本研究以其种子为外植体,附加不同浓度的6-BA、IBA、NAA、KT和AC等筛选出种子无菌萌发、原球茎增重、原球茎分化、原球茎诱导、丛生芽增殖和幼苗生根的适宜培养基；比较不同的炼苗处理方式对移栽成活率的影响；测定原球茎增重培养的动力学参数指标。以始兴石斛(4个月苗龄)成株为材料,研究了6-BA、TDZ、蔗糖、光周期和温度对诱导试管成花的影响作用,结果表明：
适合种子萌发的培养基是MS+6-BA1.5mg·L-1+NAA0.6mg·L-1+AC(活性炭)0.1%,其萌发率可达100%；适合原球茎增重的培养基是MS+6-BA0.8mg·L-1+NAA0.3mg·L-1+蔗糖4.5%,其增殖系数为7.8倍；适合分化的培养基是MS+6-BA2.5mg·L-1+NAA0.5mg·L-1+AC0.03%,其分化率为92%；...
【英文摘要】
In2010Dendrobium shixing has been found in shixing county, northern of Guangdong, and now it had been found only a few areas of Guangdong and Jiangxi border distribution, appropriate elevation of about400～600m. In recent years, the resources of the wild Dendrobium was predatory acquisition, already has been facing endangerment and extinction situation.
Used the Dendrobium shixingense seeds as explants. we select the optimal medium for germination of seeds, multiplication and differentiation of protocorm...
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