Objective To investigate the influence of L-glutamine on the expression of heat shock protein70in Muller cells in vitro.
Methods A stable method to culture Muller cells in vitro was found by dissecting the retinae of rats postnatal5-7days into cell suspension after digested by trypsin. Immunocytochemical technique was used to indentify the Muller cells, such as anti-rat glutamine synthetase (GS) antibody. Cultured Muller cells in vitro were randomly divided into normal control group, zinc sulfate group i...