关于动物行为与认知的神经支配已经成为神经科学、信息科学等交叉学科究的热点。神经元胞外记录技术的完善,使得我们能够容易提取到单个甚至同时记录到大量神经元群体的胞外锋电位发放情况,这为研究与运动相关神经元、核团及行为控制通路提供了极好的技术。
本实验以成年SD(Sprague-Dawley)大鼠为实验材料,利用自己制作的4电极(Tetrod)或2电极(Stereotrode),通过由美国Plexon公司生产的MAP 16道数据采集处理系统,记录并提取麻醉状态下大鼠尾壳核(Nucleus CaudatePutamen,CPu)及黑质网状部(Substantia nigra pars reticulate,SNr)单个神经元的锋电位序列,并结合脑内微量注射谷氨酸钠以兴奋大脑初级运动皮层(primary motor cortex,M1)及CPu的刺激方法,研究了M1-CPu和CPu-SNr通路中相关核团的作用关系,以期能够对以神经控制为基础的动物机器人的运动制导提供理论基础。
实验发现:(1)麻醉状态下大鼠大多数CPu神经元为爆发式放电,峰间隔集中分布在4-10ms内。(2)大鼠在麻醉状态下,向M1注射谷氨...
【英文摘要】
Researches about biological behavior and cognition have already become a mainattention between neural-biology and computer and information science. Extracellularrecording technology allows us to extract the extracellular spikes of a single or even alarge number of neural populations. It provides us an excellent technology forresearch on movement-related neurons, nuclei and behavioral control pathways.
In this experiment,adult SD rats were used as experimental materials. Then weused self-made tetrode or s...