Strawberry is a kind of perennial herbaceous plant in the Rosaceae family, which is a major berry crop around the world. It has highly edible and economical value. We firstly got haploidy by culturing free microspores of strawberry and optimized the inducing system in this paper. At the same time, we studied on the way to inductio the strawberry in two different methods, including colchicines induced vitro leaves and colchicines induced shoot tip meristem. We got doubling lines from both of these methods, w...