Using the the Neofinetia richardsiana Christenson seeds as explants, we select the suitable medium for germination of seeds, proliferation and differentiation of PLB,also select the suitable medium for rooting of the plant in vitro. Then compared the different methods of acclimatization, disinfection time of matrix and matrix ratio on survival rate of plantlets, and determinate all kinds of kinetic parameters changes of suspension cultures of PLB. Observed the morphology of seed germination process. The res...