Objectives
To construct human apolipoprotein O (apoO) expression vector and obtain recombinant fusion protein thioredoxin (trx)-apoO by Escherichia coli prokaryotic expression system.
Methods
The ApoO gene fragment from the human liver cDNA library was amplified by PCR. The resulting product was inserted into pET-32a vector, cloned into plasmid E. coli DH10B and sequenced after double digestion. The confirmed cDNA then transformed into E. coli BL 21 (DE3) where it was induced to express protein ...
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