From the wheat salt tolerance mutant RH8706-49 Which have been achieved via anther cultivating and EMS induced mutation from one seed in our lab, our lab had cloned the gene gs2 (Glutamine synthase2). In this test we construct the binary express vector pl300-gs2, With the control vector pl3004, we transferred the two plasmids (pl300-gs2 and pl300) into agrobactrium GV3101 and got the positive clones with the screening of hygromycin, Using the method of floral dip with the screening of hygromycin we g...
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