The thesis includes four parts: The introduction; Detection of single antibody molecules in solution by total internal reflection fluorescence microscopy; Detection of single molecules fixed on the silanized ITO by total internal reflection fluorescence microscopy; The preliminary study of electric transfer using ITO.
In the introduction, we intrduced western blot, single molecule detection, and the usage of ITO. First, the development and apply of western blot. Second we introduce the applicati...
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