Objective: To establish the reliable method of primary culture and identification of mice muscle satellite cell in vitro.
Methods: Mice muscle satellite cell were obtained from posterior limb skeletal muscle. Then made cell suspension. The cells were separated by Percoll method and purified with different speed adherence method. Cells were observed under inverted microscope and then drawed cell growthing curve. Satellite cells were identified with monoclonal antibody against desmin and α-actin.
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