In my thesis, restorer line of SHUZHA No.9which is a new sort of hybrid rape(brassica napus) is used as my experimental material .The factors which influence the effect of the transformation are discussed, including the factors like pre-culture, seed age, concentration of hormone, etc. The plants are transformed by Agrobacterium tumefaciens-mediation method, and during the induction and differentiation, some of them are proved by PCR and Southern-blotting. Some transgenic plants and their offspring show hig...
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