在高等植物体内存在一类类似于酵母和动物体内钙调磷酸酶B的蛋白,简称CBL,它的靶蛋白是一类具有丝氨酸/苏氨酸激酶活性的蛋白,被称为CIPK(CBL-interacting protein kinase)。最近的研究表明,CBLs与CIPKs家族可能在植物对逆境应答的信号转导过程中起重要作用。
在我们实验室前期工作中,发现了一个受水分胁迫诱导表达的CIPK基因,暂时命名为ZmCIPK1,为了更好的理解ZmCIPK1在逆境应答中的作用,我们首先利用RACE和RT-PCR方法克隆到ZmCIPK1基因,扩增cDNA全长为1652bp,5’端非翻译区36bp,编码区1416bp,3’端非翻译区200bp,推测ZmCIPK1编码471个氨基酸,分子量为51KDa。利用生物信息学技术对ZmCIPK1氨基酸序列分析发现ZmCIPK1蛋白N端22-282位是蛋白激酶区,其中22-113位是酪氨酸激酶区,C端是含有NAF结构域和PPI结构域的激酶调控区。亚细胞定位预测表明该蛋白可能是一个质膜蛋白。
Southern吸印/杂交表明ZmCIPK1在玉米基因组中以两个copy形式...
【英文摘要】
A new type of Ca~(2+) sensors unique to higher plants have been studied recently, they are the so-called CBLs for their similarity to B-subunit of yeast and animal calcineurin. Plant CBLs interact with and regulate the activity of a group of Ser/Thr protein kinases, referred to as CIPKs .The analysis of CBLs and CIPKs families revealed that they play an important role in the stress signal transduction pathway of higher plants.In our previous studies, we found a CIPK gene induced by water stress in maize, na...