Both prokaryotes and eukaryotes exhibit a cold-shock response upon an abrupttemperature downshift. About 26-27 cold-shock proteins are synthesized to overcome thedeleterious effects of low temperature stress. CspA, the major cold-shock protein ofEscherichia coli, has recently been studied with respect to its structure, function andregulation at the level of transcription, translation and mRNA stability, especially the functionof 5’-UTR of its mRNA.
Overexpression of proteins in E. coli at low tem...