The stable, efficient plant regeneration system is the prerequisite to success for transformation with Agrobacterium tumefaciens. In this study, the shoot tips isolated from 'Toyonoka' , 'Zoji' , and 'Shuang20' were used as explants. By means of the orthogonal experiment design, analysis of variance and multiple comparisons, the factors including genotypes, basic media, plant growth regulators ,dark periods, AgNO3 concentrations and antibiotics were analyzed and the ideal regeneration system of strawberry (...