Lactase can be used for treatment of lactose intolerance and production of low-lactose milk, whey and other dairy products. Main problems of lactase production are low yield, complicate process of deriving from yeasts and narrow applied range.
The lacO gene encoding a Galactosidase from Bacillus circulansand was modified to the lacM gene without changing its amino acid sequence. According to the bias in codon choice of yeast and the factors which affect the gene transcription, translation and the ...