In this research study, tissue culture of Torreya grandis. was investigated which includes callus induction, adventitious shoot regeneration and somatic embryogenesis. Plantlets were obtained for the first time through somatic embryogenesis.
Various explants have different callus induction rate. From leaf, stem, bud , aril and immature embryo 79.1 % 80.6% 77.1 % 68.9% and 69.8 % callus was induced. Callus from leaf, stem and aril was not stable and couldn't proliferate on subsequ...